Journal: Polymers

Article Title: Influence of DNA Type on the Physicochemical and Biological Properties of Polyplexes Based on Star Polymers Bearing Different Amino Functionalities

doi: 10.3390/polym15040894

Figure Lengend Snippet: Size distribution curves from DLS of star-shaped PDMAEMA polymers in water at 25 °C and 100 μg.mL −1 ( a ). pH versus volume of 0.1 M HCl curves for star-shaped PDMAEMA polymers ( b ). The titration was performed at 25 °C and initial concentration and volume of 500 μg.mL −1 and 3 mL, respectively.

Article Snippet: In detail, MG63 was cultured in DMEM/F-12 with GlutaMAX (Gibco, Langley, OK, USA), 10% Fetal Bovine Serum (FBS, Gibco) and 1% penicillin-streptomycin (pen/strep, 100 U.mL −1 –100 µg.mL −1 ); hFOBs were cultured in DMEM/F-12 without phenol red (Gibco), with 10% Fetal Bovine Serum (FBS, Gibco) and 0.3 mg.mL −1 Geneticin (G418, Gibco); MSCs were cultured in Alpha-MEM Glutamax (Gibco), 15% Fetal Bovine Serum (FBS, Gibco), 10 ng.mL −1 recombinant human FGF-basic (Gibco) and 1% penicillin-streptomycin (pen/strep, 100 U.mL −1 –100 µg.mL −1 ).

Techniques: Titration, Concentration Assay

Journal: Polymers

Article Title: Influence of DNA Type on the Physicochemical and Biological Properties of Polyplexes Based on Star Polymers Bearing Different Amino Functionalities

doi: 10.3390/polym15040894

Figure Lengend Snippet: Cell viability analysis. MTT assay was performed after 24 h, 48 h and 72 h of being in the culture. MSCs ( A ), hFOBs ( B ) and MG63 ( C ) were cultured in contact with 5 concentrations of AF-P/lDNA 115, AF-Q/lDNA 115, AF-P/lDNA 2000 and AF-Q/lDNA 2000 (5 µg.mL −1 ; 10 µg.mL −1 ; 20 µg.mL −1 ; 25 µg.mL −1 ; 40 µg.mL −1 ; 50 µg.mL −1 ) at N/P of 8. The data show the percentage of viable cells as compared to cells only as the control, and the mean ± SEM are presented. * p value < 0.05, *** p value < 0.001, **** p value < 0.0001.

Article Snippet: In detail, MG63 was cultured in DMEM/F-12 with GlutaMAX (Gibco, Langley, OK, USA), 10% Fetal Bovine Serum (FBS, Gibco) and 1% penicillin-streptomycin (pen/strep, 100 U.mL −1 –100 µg.mL −1 ); hFOBs were cultured in DMEM/F-12 without phenol red (Gibco), with 10% Fetal Bovine Serum (FBS, Gibco) and 0.3 mg.mL −1 Geneticin (G418, Gibco); MSCs were cultured in Alpha-MEM Glutamax (Gibco), 15% Fetal Bovine Serum (FBS, Gibco), 10 ng.mL −1 recombinant human FGF-basic (Gibco) and 1% penicillin-streptomycin (pen/strep, 100 U.mL −1 –100 µg.mL −1 ).

Techniques: MTT Assay, Cell Culture

Journal: Polymers

Article Title: Influence of DNA Type on the Physicochemical and Biological Properties of Polyplexes Based on Star Polymers Bearing Different Amino Functionalities

doi: 10.3390/polym15040894

Figure Lengend Snippet: Morphological analysis of MSCs, hFOBs and MG63 cultured in presence of 20 µg.mL −1 of polyplexes at N/P of 8 for 24 h. Microspheres evaluated by phalloidin staining (green: actin filaments; blue: cell nuclei). Insets: details at higher magnification. Scale bars: 100 µm.

Article Snippet: In detail, MG63 was cultured in DMEM/F-12 with GlutaMAX (Gibco, Langley, OK, USA), 10% Fetal Bovine Serum (FBS, Gibco) and 1% penicillin-streptomycin (pen/strep, 100 U.mL −1 –100 µg.mL −1 ); hFOBs were cultured in DMEM/F-12 without phenol red (Gibco), with 10% Fetal Bovine Serum (FBS, Gibco) and 0.3 mg.mL −1 Geneticin (G418, Gibco); MSCs were cultured in Alpha-MEM Glutamax (Gibco), 15% Fetal Bovine Serum (FBS, Gibco), 10 ng.mL −1 recombinant human FGF-basic (Gibco) and 1% penicillin-streptomycin (pen/strep, 100 U.mL −1 –100 µg.mL −1 ).

Techniques: Cell Culture, Staining

Journal: Polymers

Article Title: Influence of DNA Type on the Physicochemical and Biological Properties of Polyplexes Based on Star Polymers Bearing Different Amino Functionalities

doi: 10.3390/polym15040894

Figure Lengend Snippet: ( a ) Cells viability analysis. MTT assay was performed after 24 h of culture. hFOBs and MG63 were cultured in contact with 20 µg.mL −1 of AF-P eGFP and AF-Q eGFP at N/P of 8. The data show the percentage of viable cells as compared to cells only, as the control and the mean ± SEM are presented. ( b ) Qualitative transfection efficacy images. eGFP positive cells in green and cell nuclei in blue. Scale bars: 100 µm. ( c ) Transfection efficiency. % of transfection efficiency of AF-P eGFP complex at N/P of 8 compared to Lipofectamine used as control group.

Article Snippet: In detail, MG63 was cultured in DMEM/F-12 with GlutaMAX (Gibco, Langley, OK, USA), 10% Fetal Bovine Serum (FBS, Gibco) and 1% penicillin-streptomycin (pen/strep, 100 U.mL −1 –100 µg.mL −1 ); hFOBs were cultured in DMEM/F-12 without phenol red (Gibco), with 10% Fetal Bovine Serum (FBS, Gibco) and 0.3 mg.mL −1 Geneticin (G418, Gibco); MSCs were cultured in Alpha-MEM Glutamax (Gibco), 15% Fetal Bovine Serum (FBS, Gibco), 10 ng.mL −1 recombinant human FGF-basic (Gibco) and 1% penicillin-streptomycin (pen/strep, 100 U.mL −1 –100 µg.mL −1 ).

Techniques: MTT Assay, Cell Culture, Transfection